Reporter

Part:BBa_K354002:Design

Designed by: Gregory Meyer   Group: iGEM10_WITS-South_Africa   (2010-10-20)


Venus - Fluorescent Reporter Protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 649
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence had to be codon optimised and a ribosome binding site was added to the 5' end so as to ensure translation.


Source

Venus's sequence was derived from Genbank and was codon optimised so as to yield optimal expression in Lactobacillus gasseri.

References